{"id":1241,"date":"2016-04-26T12:02:09","date_gmt":"2016-04-26T09:02:09","guid":{"rendered":"http:\/\/www.bio21.bas.bg\/ippg\/en\/?page_id=1241"},"modified":"2026-05-12T10:58:49","modified_gmt":"2026-05-12T08:58:49","slug":"plant-soil-interactions-scientific-achievements-research-team-1","status":"publish","type":"page","link":"http:\/\/www.bio21.bas.bg\/ippg\/en\/?page_id=1241","title":{"rendered":"&#8220;PLANT-SOIL INTERACTIONS&#8221; &#8211; Scientific Achievements &#8211; Research Group 2"},"content":{"rendered":"<h4 style=\"text-align: justify;\">Research Group<\/h4>\n<h5 style=\"text-align: justify;\">Development of protocols for micropropagation of endangered, traditional and nontraditional for bulgaria medicinal and aromatic plants<\/h5>\n<p style=\"text-align: justify;\">Joint development of participants from the Laboratory &#8220;Plant-Soil Interactions&#8221; and the Laboratory &#8220;Regulators of Plant Growth and Development&#8221;<\/p>\n<p style=\"text-align: justify;\">Propagation of <em>Stevia rebaudiana<\/em> Bertoni. is limited by low seed viability and germination, and poor rooting of vegetative cuttings. Therefore, an alternative biotechnological method for its propagation was studied, using direct organogenesis with the addition to the nutrient medium of nanofibers formed from amino acid valine derivatives, as carriers of the biologically active agent silver atoms\/particles (NF-1%Ag and NF-2%Ag). treatment with the nanofibers stimulated the growth parameters, and the antioxidant activity in <em>S. rebaudiana<\/em>. The total sugar content in plantlets increased in plants treated with NF-1%Ag and NF-2%Ag compared with untreated control plants and those grown on cytokinin medium. The amounts of the sweet diterpene glycosides \u201cstevioside\u201d and \u201crebaudioside A\u201d in stevia plantlets increased more significantly in plants treated with NF-1%Ag. The steviosides synthesized in the leaves of <em>S. rebaudiana<\/em> are 300 times sweeter than regular table sugar, have zero calories and carbohydrates, and do not cause an increase in blood sugar levels. Nowadays, the food industry is focusing on replacing artificial sweeteners with natural sugars, therefore the optimized protocol for <em>in vitro<\/em> <em>S. rebaudiana <\/em>propagation may find application in practice.<\/p>\n<p style=\"text-align: justify;\">Scientific publication:<\/p>\n<p style=\"text-align: justify;\">Sichanova M; Geneva M*; Petrova M; Miladinova-Georgieva K; Kirova E; Nedev T; Tsekova D; Iwanov I; Dochev K; Ivanova V; Trendafilova A, Improvement of <em>Stevia rebaudiana <\/em>Bertoni in vitro propagation and steviol glycoside content using amino acid silver nanofibers, Plants 2022, 11(19), 2468, https:\/\/doi.org\/10.3390\/plants11192468\u0440, Q1, IF: 4.658<\/p>\n<div id=\"attachment_3646\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3646\" class=\"wp-image-3646\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2026\/05\/PSI_Fig-2-1-150x150.jpg\" alt=\"\" width=\"500\" height=\"240\" srcset=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2026\/05\/PSI_Fig-2-1-300x144.jpg 300w, http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2026\/05\/PSI_Fig-2-1.jpg 602w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><p id=\"caption-attachment-3646\" class=\"wp-caption-text\">\u0424\u0438\u0433. 1. \u0410) Stevia rebaudiana Bertoni in vitro propagated; B) Content of metabolites with antioxidant potential in Stevia rebaudiana plantlets grown in vitro on medium enriched with different concentrations of amino acid nanofibers carriers of 1% and 2% colloidal Ag.<\/p><\/div>\n<hr \/>\n<h6 style=\"text-align: right;\"><a class=\"_ps2id\" href=\"#top\" data-ps2id-offset=\"300\">[ Top ]<\/a><\/h6>\n","protected":false},"excerpt":{"rendered":"<p>Research Group Development of protocols for micropropagation of endangered, traditional and nontraditional for bulgaria medicinal and aromatic plants Joint development of participants from the Laboratory &#8220;Plant-Soil Interactions&#8221; and the Laboratory &#8220;Regulators of Plant Growth and Development&#8221; Propagation of Stevia rebaudiana Bertoni. is limited by low seed viability and germination, and poor rooting of vegetative cuttings. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":1226,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/1241"}],"collection":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1241"}],"version-history":[{"count":17,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/1241\/revisions"}],"predecessor-version":[{"id":1249,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/1241\/revisions\/1249"}],"up":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/1226"}],"wp:attachment":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1241"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}