{"id":2325,"date":"2020-06-02T11:03:52","date_gmt":"2020-06-02T09:03:52","guid":{"rendered":"http:\/\/www.bio21.bas.bg\/ippg\/en\/?page_id=2325"},"modified":"2025-12-08T10:22:40","modified_gmt":"2025-12-08T08:22:40","slug":"regulators-of-plant-growth-and-development-scientific-achievements","status":"publish","type":"page","link":"http:\/\/www.bio21.bas.bg\/ippg\/en\/?page_id=2325","title":{"rendered":"REGULATORS OF PLANT GROWTH AND DEVELOPMENT &#8211; Scientific Achievements"},"content":{"rendered":"<h5 style=\"text-align: justify;\">Investigation of the role of growth regulators of synthetic and natural origin and other biologically active substances on the growth and development of plants under optimal and stressful conditions. Study of the natural and induced protective responses of different plant species to abiotic and biotic stressors. Application of newly established or known growth regulators as a means to increase the resistance of economically important plants to adverse environmental conditions such as soil drought, waterlogging, salinization, heavy metal pollution, high and low temperatures, UV radiation, herbicides, phytopathogens and other stresses.<\/h5>\n<p style=\"text-align: justify;\">The physiological responses of wheat to drought and soil waterlogging after routine application of the selective herbicide Zerrate (Syngenta) were studied using biochemical, biophysical and molecular biological analyses. It was found that treatment with the herbicide and subsequent soil drought for 7 days did not further damage the wheat and the plants recovered successfully after resumption of watering. When treated with the herbicide and seven-day waterlogging, the physiological disorders deepened and the plants failed to recover.<\/p>\n<p style=\"text-align: justify;\">In triticale, the herbicide applied in combination with drought further increased the accumulation of reactive oxygen species, which enhanced the damage and induced a greater activity of antioxidant- and xenobiotic-detoxifying enzymes. In combination with waterlogging, the herbicide did not cause additional damage in the plants.<\/p>\n<div id=\"attachment_3469\" style=\"width: 610px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3469\" class=\"wp-image-3469\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Serrate_Wh-Tr-150x150.png\" alt=\"\" width=\"600\" height=\"519\" srcset=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Serrate_Wh-Tr-300x259.png 300w, http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Serrate_Wh-Tr-1024x885.png 1024w, http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Serrate_Wh-Tr-768x664.png 768w, http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Serrate_Wh-Tr.png 1460w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><p id=\"caption-attachment-3469\" class=\"wp-caption-text\">Phenotypic alterations of wheat plants treated with herbicide and subjected to drought or flooding stress. C \u2013 Control; H \u2013 Herbicide; D \u2013 Drought; H+D \u2013 Herbicide + Drought; F \u2013 Flooding; H+F \u2013 Herbicide + Flooding.<br \/><a href=\"https:\/\/doi.org\/10.3390\/plants10040733\" target=\"_blank\" rel=\"noopener noreferrer\">Todorova et al. (2021) Plants, 10(4), 733-745<\/a> <br \/><a href=\"https:\/\/doi.org\/10.3390\/plants10061195\" target=\"_blank\" rel=\"noopener noreferrer\">Katerova et al. (2021) Plants, 10(6), 1195-1206<\/a> <br \/><a href=\"https:\/\/doi.org\/10.3390\/agronomy12020390\" target=\"_blank\" rel=\"noopener noreferrer\">Todorova et al. (2022) Agronomy, 12(2), 390-403<\/a><\/p><\/div>\n<hr \/>\n<p style=\"text-align: justify;\">For a first time, a protective effect of two types of synthetic analogues of the plant hormone auxin on the physiological state of pea plants in drought conditions has been established. Under normal growing conditions, the auxin compounds did not significantly change the biometric and biochemical parameters of the plants. Under drought conditions, auxin analogues reduced the negative effects of stress, causing changes in physiological and metabolic processes, which were expressed as lower content of stress biomarkers and improved growth of pea plants. The proven protective effect of the tested compounds is a prerequisite for their potential practical application.<\/p>\n<div id=\"attachment_11314\" style=\"width: 425px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11314\" class=\"wp-image-11314\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Fig-TA_1.jpg\" alt=\"\" width=\"415\" height=\"450\" \/><p id=\"caption-attachment-11314\" class=\"wp-caption-text\">Exogenous auxin type compounds amend PEG-induced physiological responses of pea plants.<br \/><a href=\"https:\/\/doi.org\/10.1016\/j.scienta.2019.01.015\" target=\"_blank\" rel=\"noopener noreferrer\">Sergiev et al. (2019) Sci. Horticult., 248, 200-205<\/a><\/p><\/div>\n<p style=\"text-align: justify;\">Pre-treatment with synthetic auxins partially improved the growth of pea plants treated with herbicides. This is accompanied by a reduction in non-enzymatic antioxidants and stress markers. Pre-treatment with auxin compounds specifically modulated the activity of key antioxidant enzymes, stimulated endogenous plant protection and reduced the negative effects of herbicidal action.<\/p>\n<div id=\"attachment_11323\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11323\" class=\"wp-image-11323\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Fig-1_TA.jpg\" alt=\"\" width=\"500\" height=\"263\" \/><p id=\"caption-attachment-11323\" class=\"wp-caption-text\">Phenotypic changes of 28-day-old pea plants sprayed with TA-12 or TA-14 and subsequently treated with Glyphosate or Glean-75.<br \/><a href=\"https:\/\/doi.org\/10.2478\/s11756-020-00557-0\" target=\"_blank\" rel=\"noopener noreferrer\">Sergiev et al. (2020) Biologia, 75, 1845-1853<\/a><\/p><\/div>\n<hr \/>\n<p style=\"text-align: justify;\">The protective effect of \u03b2-monomethyl ester of itaconic acid (MEIA) against UV-B radiation has been proven in tomato. After UV-B treatment typical phenotypic alteratons, such as drying and wilting of the leaves, were observed in tomato cv. Ailsa Craig and its anthocyaninless isogenic line ah (anthocyaninless of Hoffman). These effects were more pronounced in the anthocyaninless mutant ah. Pre-treatment of MEIA reduced the negative effects of UV-B, and the positive effects of MEIA was significantly stronger in ACr genotype.<\/p>\n<div id=\"attachment_11334\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11334\" class=\"wp-image-11334\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Fig-2_Tomato_UV.jpg\" alt=\"\" width=\"500\" height=\"432\" \/><p id=\"caption-attachment-11334\" class=\"wp-caption-text\">Phenotypic changes of ACr and ah tomato plants preliminary treated with 1 mM \u03b2-monomethyl ester of itaconic acid (MEIA) and irradiated with 12.8 kJ m<sup>\u22122<\/sup> day<sup>\u22121<\/sup> UV-B light at the 48th hour after irradiation.<br \/><a href=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Katerova-et-al_2014-4.pdf\">Katerova et al. (2014) Compt. Rend. Acad. Bulg. Sci., 67(4), 533-540<\/a><\/p><\/div>\n<hr \/>\n<p style=\"text-align: justify;\">For the first time, the effect of the application of the lytic bacteriophage BsXeu269p\/3 isolated in Bulgaria on pepper plants (<em>Capsicum annuum<\/em> L.) infected with <em>Xanthomonas euvesicatoria<\/em> 269p &#8211; the main causative agent of bacteriosis in the country &#8211; has been studied. Completely new and original methodological approaches have been developed for the detailed study of the pepper-bacterium-bacteriophage pathosystem. The results obtained prove that the <em>in vivo<\/em> application of BsXeu269p\/3 leads to an interruption in the transmission of infection between infected and healthy plants, a reduction in the symptoms of the disease and a fivefold decrease in the amount of the phytopathogen in infected plants. These results can serve as a basis for its use as a means of biocontrol in practice.<\/p>\n<div id=\"attachment_3471\" style=\"width: 443px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3471\" class=\"wp-image-3471 size-full\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Nauchno-pril_post.jpg\" alt=\"\" width=\"433\" height=\"511\" srcset=\"http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Nauchno-pril_post.jpg 433w, http:\/\/www.bio21.bas.bg\/ippg\/en\/wp-content\/uploads\/2025\/12\/Nauchno-pril_post-254x300.jpg 254w\" sizes=\"(max-width: 433px) 100vw, 433px\" \/><p id=\"caption-attachment-3471\" class=\"wp-caption-text\">Effect of the application of the lytic bacteriophage BsXeu269p\/3 isolated in Bulgaria on pepper plants (Capsicum annuum L.) infected with Xanthomonas euvesicatoria 269p. (A) Chlorotic spots on the leaves of pepper plants (Capsicum annuum L., cultivar Sofia Kapia) inoculated with Xanthomonas euvesicatoria 269p by point infection; (B) Reduction of visible symptoms in the same leaf after treatment with BsXeu269p\/3, 11 days after infection; (C) Epiphytic colony of Xanthomonas euvesicatoria 269p on the lower surface of a pepper leaf, seven days after infection; (D) The potential bacteriolytic effect of BsXeu269p\/3 on the same bacterium in plant samples. Photographs (C) and (D) were taken on a scanning electron microscope.<br \/><a href=\"https:\/\/doi.org\/10.3390\/plants12193348\">Shopova et al. (2023) Plants, 12, 19<\/a><\/p><\/div>\n<hr \/>\n<p style=\"text-align: justify;\">It was found that foliar treatment with the polyamine spermine reduced the adverse effects of soil salinity in young plants of <em>Salvia officinalis<\/em> L. Salinity provoked inhibition of sage growth, accompanied by a loss of turgor and reduction of rosemarinic and carnosic acids. Pre-treatment with spermine reduced the effects of salinity on the measured parameters and increased the content of the secondary metabolites studied.<\/p>\n<div id=\"attachment_11353\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11353\" class=\"wp-image-11353\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Salvia.jpg\" alt=\"\" width=\"500\" height=\"227\" \/><p id=\"caption-attachment-11353\" class=\"wp-caption-text\">Effects of spermine (Spm), NaCl and combination of them on <em>Salvia officinalis<\/em> L.<br \/><a href=\"http:\/\/www.proceedings.bas.bg\/DOI\/doi2020_6_07.html\" target=\"_blank\" rel=\"noopener noreferrer\">Todorova et al. (2020) Compt. Rend. Acad. Bulg. Sci., 73(6), 800-808<\/a><\/p><\/div>\n<hr \/>\n<p style=\"text-align: justify;\">We studied the effects of plant growth regulators with different mechanisms of action &#8211; phenylurea cytokinin (4PU-30), retardant (MEIA, \u03b2-monomethyl ester of itaconic acid) and triacontanol (TRIA, higher aliphatic alcohol) &#8211; on young tomato and pepper plants, infected with <em>Tomato spotted wilt virus<\/em> (TSWV). The treatment with plant growth regulators was performed before or after virus infection. The level of TSWV infection was quantified by DAS-ELISA. MEIA was found to inhibit the development of TSWV infection in two tomato lines Keti and VK1, while 4PU-30 was effective only in the Keti line, which is more sensitive to TSWV than the VK1 line. TRIA reduced the optical density of TSWV, and its effect was better when applied before infection.<\/p>\n<div id=\"attachment_11359\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11359\" class=\"wp-image-11359 size-full\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Moskova-et-al-2.jpg\" alt=\"\" width=\"500\" height=\"172\" \/><p id=\"caption-attachment-11359\" class=\"wp-caption-text\">Effects of MEIA and 4PU-30 on the optical density (\u041eD) of TSWV, represented by the average extinction values for TSWV in non-infected (\u2013TSWV) and infected (+TSWV) with TSWV tomato lines \u201cKeti\u201d and VK1.<br \/><a href=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Moskova_et-al_CRB_2020-73-11.pdf\">Moskova et al. (2020) Compt. Rend. Acad. Bulg. Sci., 73(11), 1535-1544<\/a><\/p><\/div>\n<div id=\"attachment_11356\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11356\" class=\"wp-image-11356\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Moskova-et-al.jpg\" alt=\"\" width=\"500\" height=\"327\" \/><p id=\"caption-attachment-11356\" class=\"wp-caption-text\">Effects of Tomato spotted wilt virus (TSWV) and triacontanol (TRIA) on pepper plants: A \u2013 infected with TSWV. Two infected plants and one healthy plant are shown. B \u2013 effects of TRIA on infected pepper plants.<br \/><a href=\"http:\/\/www.proceedings.bas.bg\/DOI\/doi2021_7_17.html\" target=\"blank\" rel=\"noopener noreferrer\">Moskova et al. (2021) Compt. Rend. Acad. Bulg. Sci., 74(7), 1089-1095<\/a><\/p><\/div>\n<hr \/>\n<h5 style=\"text-align: justify;\">Development of innovative <em>in vitro<\/em> models based on biotechnology and bioinformatics and their use in studying the action of regulators of growth and development of plants under normal and stress, to increase productivity, to stimulate the biosynthesis of secondary metabolites for nutraceutics, to preserve gene pool by conserving rare and endangered species.<\/h5>\n<p style=\"text-align: justify;\"><em>In vitro<\/em> cultures of important agricultural, medicinal and aromatic plants were obtained through developed protocols for regeneration, micropropagation and long-term callus and organogenic cultures of pea, soybean, <em>Arnica montana<\/em> L. (mountain arnica), <em>Sideritis scardica<\/em> Gris (Mursal tea), <em>Rhodiola rosea<\/em> L. (golden root), <em>Rhodiola kirilowii<\/em> (Regel) Maxim., <em>Melissa officinalis<\/em> L. (balm), <em>Crocus sativus<\/em> L. (saffron crocus) and others. Their ability to synthesize substances with antioxidant and protective properties has been proven. The developed <em>in vitro<\/em> technologies could find application for the production of plants for commercial purposes and sustainable conservation of endangered species.<\/p>\n<div style=\"width: 460px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2011\/03\/rrrr_f3.jpg\"><img loading=\"lazy\" decoding=\"async\" title=\"rrrr_f3\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2011\/03\/rrrr_f3.jpg\" alt=\"\" width=\"450\" height=\"348\" \/><\/a><p class=\"wp-caption-text\">Induced rhizogenesis and propagation in <em>Rhodiola rosea<\/em> regenerated plants on MS medium enriched with 2.0 mg\/L IBA, 0.2 mg\/L IAA, and 0.4 mg\/L GA<sub>3<\/sub>.<br \/><a href=\"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11535-010-0092-3\/html\" target=\"_blank\" rel=\"noopener noreferrer\">Tasheva &amp; Kosturkova (2010) Cent. Eur. J. Biol. 5(6), 853-863<\/a><\/p><\/div>\n<div id=\"attachment_11295\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11295\" class=\"wp-image-11295\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Arnica.jpg\" alt=\"\" width=\"500\" height=\"334\" \/><p id=\"caption-attachment-11295\" class=\"wp-caption-text\"><span id=\"page11R_mcid421\" class=\"markedContent\"><span dir=\"ltr\" role=\"presentation\">Multiplied Shoots Developed on MS Medium with Different Composition: <\/span><\/span> \u0430) MS + 1mg\/l BAP \u0438 0.1 mg\/l IAA; b) MS + 1mg\/l Zeatin \u0438 0.1 mg\/l IAA, b) MS + 1mg\/l 2-iP \u0438 0.1 mg\/l IAA.<br \/><a href=\"https:\/\/hrcak.srce.hr\/en\/255028\" target=\"_blank\" rel=\"noopener noreferrer\">Petrova et al. (2021) Agric. Consp. Sci., 86(1), 57-65<\/a><\/p><\/div>\n<div id=\"attachment_11297\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11297\" class=\"wp-image-11297\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Arnica-1.jpg\" alt=\"\" width=\"500\" height=\"130\" \/><p id=\"caption-attachment-11297\" class=\"wp-caption-text\"><em>In vitro<\/em> rooting and acclimatization of <em>Arnica montana<\/em>: a) <em>In vitro<\/em> rooting of <em>A. montana<\/em> on \u00bd MS with 0.5 mg\/l IBA; b) <em>Ex vitro<\/em> adapted plants; c) Plants cultured in the experimental fi\u0435ld \u201cBeglika\u201d; d) Three-year old plants during flowering stage.<br \/><a href=\"https:\/\/hrcak.srce.hr\/en\/255028\" target=\"_blank\" rel=\"noopener noreferrer\">Petrova et al. (2021) Agric. Consp. Sci., 86(1), 57-65<\/a><\/p><\/div>\n<div id=\"attachment_11300\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-11300\" class=\"wp-image-11300\" src=\"http:\/\/www.bio21.bas.bg\/ippg\/bg\/wp-content\/uploads\/2022\/03\/Melissa.jpg\" alt=\"\" width=\"500\" height=\"256\" \/><p id=\"caption-attachment-11300\" class=\"wp-caption-text\"><em>In vitro<\/em> cultivation of <em>Melissa officinalis<\/em>: Micropropagation on MP3 medium (MS supplemented with 1.5 mg\/L BAP and 0.5 mg\/LNAA:a) first subculture) and b) second subculture; c) <em>In vitro<\/em> rooting on \u00bd MS nutrient medium; d) <em>Ex vitro<\/em> adapted plants.<br \/><a href=\"https:\/\/office2.jmbfs.org\/index.php\/JMBFS\/article\/view\/4077\" target=\"_blank\" rel=\"noopener noreferrer\">Petrova et al. (2021) J. Microbiol. Biotechnol. &amp; Food Sci., 11(3), e4077<\/a><\/p><\/div>\n<hr \/>\n<h6 style=\"text-align: right;\"><a class=\"_ps2id\" href=\"#top\" data-ps2id-offset=\"300\"> \u2191 Top \u2191<\/a><\/h6>\n","protected":false},"excerpt":{"rendered":"<p>Investigation of the role of growth regulators of synthetic and natural origin and other biologically active substances on the growth and development of plants under optimal and stressful conditions. Study of the natural and induced protective responses of different plant species to abiotic and biotic stressors. Application of newly established or known growth regulators as [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":251,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/2325"}],"collection":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2325"}],"version-history":[{"count":21,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/2325\/revisions"}],"predecessor-version":[{"id":2722,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/2325\/revisions\/2722"}],"up":[{"embeddable":true,"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=\/wp\/v2\/pages\/251"}],"wp:attachment":[{"href":"http:\/\/www.bio21.bas.bg\/ippg\/en\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}