A fast and reliable method to measure the activity
of photosynthetic herbicides in intact plants

Analysis of the Results

Dose-response curves

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Fig. 15. Dose-response curve.

The dose-response relationship can be obtained by measuring the effect of the herbicide (response) at a set of different concentrations (doses). As a measure of the effect we use the amplitude of the I4 peak of the DFIC. The dose-response curve can be approximated to a sigmoidal function whose upper assymptote corresponds to untreated samples and the lower assymptote to samples with fully inhibited electron transport.

The dose-response curve provides a mean to estimate the concentration of half-inhibition, or its negative logarithm - the index of half-inhibition, pI50. The higher pI50 is, the more sensitive is the plant towards the given herbicide (or the more effective is the herbicide in the given plant).

 

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