Applied genetics and plant biotechnologies

Assoc. Prof. E. Zayova

Leader

Associate Professor Dr Ely Zayova

Phone: +359-2-9746229 int. 355
E-mail: e_l_y@abv.bg

Personal page

FIELD OF RESEARCH
  • Development and evaluation of cultivated sunflower (Helianthus annuus L.), tomatoes (Solanum lycopersicum L.), pepper (Capsicum annuum L.), maize (Zea mays L.), tobacco (Nicotiana tabacum L.), wheat (Triticum aestivum L.), coriander (Coriandrum sativum L.), dill (Anethum graveolens L.), fennel (Foeniculum vulgare Mill.), caraway (Carum carvi L.) etc., with valuable agronomic and biological traits – resistance to biotic and abiotic stress, productivity, disease resistant, high food quality, early maturity etc.
  • Development of initial plant material for plant breeding and genetics.
  • Population variability of phytopathogens and induced resistance in plants.
  • Finding sources of complex resistance to economically important diseases in cultivated plants.
  • Micro propagation of endangered, traditional and nontraditional medicinal species and obtaining suitable plant material for breeding programs.
  • Gene transfer and application of chemical agents for increasing the synthesis of secondary metabolites in medicinal plants.
RESEARCH PROJECTS
  • Exploration of Balkan biodiversity of Capsicum spp. to extract biotic stress resistant germplasm, SEE-ERA.NET Plus Joint Call ERA 226/01. Project coordinator: Assoc. Prof. Dr. R. Rodeva. Partners: Maritsa Vegetable Crops Research Institute, Plovdiv; K. Malkov Institute of Plant Genetic Resources, Sadovo; Institute for Vegetable Crops, Smederevska Palanka, Serbia; Goce Delcev University – Shtip, FYR Macedonia; Plant Protection Institute of Patras – NAGREF, Greece; Agricultural University of Tirana, Albania.
  • Phenotypic and genotypic characterization of Phomopsis/Diaporthe species on cultivated and wild growing plants in Bulgaria and Lithuania, Bilateral project Bulgaria – Lithuania. Project leader: Assoc. Prof. Dr. R. Rodeva. Partners: Institute of Botany of Nature Research Centre, Vilnius, Lithuania.
  • Development of pepper germplasm resistant to some fungal diseases, Bilateral project Bulgaria – Lithuania. Project leader: Assoc. Prof. Dr. R. Rodeva. Partners: Lithuanian Institute of Horticulture of Lithuanian Centre of Agricultural and Forestry Sciences, Babtai, Kaunas reg., Lithuania.
  • Biodiversity of wheat leaf spotting fungi in European domain, NSF – Bilateral Scientific – Technical collaboration between Bulgaria and Slovakia Project leader: Assoc. Prof. Dr. R. Rodeva. Partners: Field Crop Institute – Chirpan; Plant Production Research Center Piestany, Slovakia
  • Complex study of protected, endemic and rare high-mountain medicinal plants from the Bulgarian flora – preservation and sustainable use, Project 02/38, National Science Fund, Ministry of Education, Youth and Science, Coordinator: Assoc. Prof. Dr. E. Zayova. Partners: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences.
  • Contract between the Institute of Plant Physiology and Genetics, Bulgarian Academy of Science and “Tevio” LTD, Sofia, “In vitro propagation of Stevia rebaudiana Bertoni”, Project leader: Assoc. Prof. Dr. E. Zayova
  • Contract between the National Bank for Industrial Microorganisms and Cell Cultures and the Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences. Project leader: Prof. Dr. E. Stoimenova
  • Contract between Plant Physiology and Genetics Bulgarian Academy of Sciences and Agriculture Experimental Station, Kardzhali. Project leader:  Assistant Prof. D. Stoeva
  • Project: № BG051PO001-3.3.06- 0025 entitled “Support for the creation and development of young competitive research potential in the field of physiology, phytochemistry, genomics, proteomics and biodiversity of eukaryotic organisms” with the financial support of Operational Programme “Human Resources Development”. Participants: E. Balacheva, T. Kartseva and M. Petrova.
SESECTED RESEARCH ACHIEVEMENTS
  • Two tomato mutants genes ah (Hoffmann’s anthocyaninless) and aw (anthocyanin without) are characterized by complete absence of anthocyanin in all vegetative tissues over the tomato developmental stages. Lycopene content in fruits of this anthocyaninless mutants are significantly higher that of the wild type, exceeding it by 30 %.
  • Using wide hybridization (interspecific and intergeneric crosses) novel sunflower lines possessing intermediate and even new characteristics were developed. Some of these lines exhibited valuable traits such as drought and salt tolerance, as well as fatty acid composition of the seeds. All these plant materials might be used as initial lines for including in theoretical and applied investigations for sunflower breeding programs.

Sunflower lines obtained after interspecific and intergeneric hybridization.

  • It is established that malate dehydrogenase and esterase isozymes may be used as markers for the identification of apomictic plants in the hybrid progeny of maize with its wild relative Tripsacum dactiloides. Apomictic forms are valued genetic material for breeding programs, directed for the fixture of the heterotic effect in maize.
  • The biodiversity of fungal species associated with leaf spotting of wheat was investigated in Bulgaria and Slovakia in the framework of bilateral research project between Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria and Plant Production Research Center Piestany, Slovakia. Current data were obtained for the economically important and emerging pathogens on T. aestivum, T. durum and some graminicolous grasses. A pathogen collection was created.
  • Tomato lines originating from three-genome hybrid (L. esculentum – L. chilense – L. peruvianum var. humifusum) with complex resistance to Pseudomonas syringae pv. tomato, (races R0 и R1), Xanthomonas vesicatoria (races T1, T2, T3 and pathotype РТ)  and Xanthomonas perforans were developed.
  • The changes in population diversity of tobamoviruses in tomato and pepper, connected with introduction of resistant to these viruses cultivars in agriculture were studied. The strains of tomato mosaic virus (ToMV), overcoming the tobamovirus resistant gene Tm-1 was isolated from commercial tomato cultivars. Pepper mild mottle virus (pathotype P1) and pepper mild mottle virus (pathotype P2) were identified from the introduced pepper cultivars grown in Bulgarian fields. The truly destructive epidemics of “veinal necrosis” disease have been observed periodically in field tomato. The necrotic stains of cucumber mosaic virus possessing fifth satellite RNA were identified as the causal agent of this disease.
  • The pyramiding of resistant genes to the most important diseases (phytophthora root rot, cucumber and tobacco mosaic diseases) and genes for good savor qualities, high quantity of b-carotene (provitamine A), vitamin C, reducing sugars, licopene, dry matter content, anthocyaninlessness in pepper lines was carried out. The developed pepper lines are suitable for early and middle early field production of sweet pepper and may be used by the plant breeders in pepper improvement programs.

  • Pepper collection created during the implementation of ERA 226 project included 235 local forms, situated in Albania (17), Bulgaria (134), Greece (34), Macedonia (11) and Serbia (39). Morphological and phenological observations as well as biometric measurements on the basis of 67 features were conducted according to Descriptors for Capsicum with some additions. Significant diversity of local pepper forms was established in the collected material. Fruit shape was used as a major trait for its arrangement.
  • The anthracnose disease on pepper was investigated in detail. Phenotypic, genotypic and pathogenic characterization of Colletotrichum isolates showed that the population of the causal agents of anthracnose is heterogenous and at least three species: C. acutatum, C. gloeosporioides and C. coccodes were associated with the development of the disease in Bulgaria. Among them C. coccodes was the most frequently found. On the basis of comparative morphological, cultural and molecular characteristics of C. coccodes obtained from fruits and roots it was proved that the fruit anthracnose and root rot were caused by one and the same species. One unusual Colletotrichum sp. was found belonging to the C. gloeosporioides group.
  • An important number of tomato (Trapezica, Bononia, Berika, Rayana F1, Rozalina-Rossa F1, Naslada F1) and maize (Zaharina F1) varieties possessing high nutritive and market quality and productivity were developed. The majority of these varieties are grown on large area in Bulgaria.
  • Efficient in vitro methods for enhanced micropropagation of valuable medicinal plants: Arnica montana and Gentiana lutea (endangered species), Valeriana officinalis, Origanum heracleoticum, Thymus vulgaris, Mentha piperita (traditional species for Bulgaria) and Echinacea purpurea, Stevia rebaudiana, Salvia hispanica (new, nontraditional species) were developed. That could contribute to enrichment and protection of biodiversity, application in the pharmacological, cosmetic and food industries.

Micropropagation of Arnica montana L.

Micropropagation of Stevia rebaudiana Bertoni.

  • The original protocol for increasing the micropropagation efficiency of Arnica montana by cultivation in temporary immersion system (TIS) was established, which provides a high propagation rate and the higher content of sesquiterpene lactones. The research was done with the co-operation of Institute of Biodiversity and Ecosystem Research, BAS and Institute of Organic Chemistry with Centre of Phytochemistry, BAS.

Micropropagation of Arnica montana by Temporary Immersion System (TIS RITA®).

  • By gene transfer (Agrobacterium rhisogenes ATCC 15834) fast growing untypical roots (hairy roots) culture of Arnica montana were induced as biomass for production of biologically active compounds.

Hairy roots of Arnica montana.

RESEARCH TEAM

The team of Department "Applied Genetics and Plant Biotechnology”

Ely Zayova, Associate Professor, PhD
E-mail: e_l_y@abv.bg
Phone: +359-2-974 62 29/ 355
Professional interests: Biotechnology for medicinal plants (micropropagation and conservation of valuable medicinal plants, plant regeneration, somaclonal variation, synthesis of secondary metabolites, cell culture, genetic transformation).

Elisaveta Stoimenova, Professor, PhD
E-mail: e.stoimenova@abv.bg
Phone: +359-2-974 62 29/ 280
Professional interests: Plant viruses, population variability of tobamoviruses and cucumoviruses, disease resistance of plants, systemic acquired resistance.
Research object: Solanum lycopersicum L.,  Capsicum annuum L.

Rossitza Rodeva, Associate Professor, PhD
E-mail: r.rodeva@abv.bg
Phone: +359-2-974 62 29/ 281, 282
Professional interests: Isolation, identification, in vitro growing of phytopathogenic fungi (Ascochyta, Botrytis, Colletotrichum, Fusarium, Itersonilia, Macrophomina, Phoma, Phomopsis, Pyrenophora, Rhizoctonia, Sclerotinia, Septoria, Stagonospora) and studying of their morphological, cultural, pathogenic and molecular diversity and the ability to produce phytotoxins; searching for resistance in host plants in vivo and in vitro; investigation on mechanisms and processes related with pathogenicity and defense response.

Rumiana Vassilevska-Ivanova, Associate Proffesor, PhD
E-mail: ru_vas_bg@yahoo.com
Phone: +359-2-974 62 29/ 220
Professional interests: Hybridization of cultivated sunflower with annual and perennial wild Helianthus species and/or species from related genera of Asteraceae; characterization and evaluation of the obtained putative hybrids using different investigation methods; screening of the obtained hybrid materials for marketable traits etc.
Research object: Helianthus annuus L.

Lydia Shtereva, Associate Proffesor, PhD
E-mail: ly_shterva@abv.bg
Phone: +359-2-974 62 29/ 254
Professional interests: Heterosis; in vivo and in vitro approaches in studying stress physiology and biochemistry; in vitro androgenesis and somaclonal variation.
Research object: Solanum lycopersicum L.

Boris Kraptchev, Assistant Professor, PhD
Е-mail: bkraptchev@yahoo.com
Phone: +359-2-974 62 29/ 209
Professional interests: Heterosis; sources of haploidy in maize; heterofertilization; apomixis.
Research object: Zea mays L.

Elena Balatcheva, Assistant Professor, PhD
Е-mail: leni_bal@yahoo.com
Phone: +359-2-974 62 29/ 205
Professional interests: Hheterosis; pleiotropic effect of mutant genes, food quality of the tomato fruit; tolerance to abiotic stress.
Research object: Solanum lycopersicum L

Maria Petrova, Assistant Professor
E-mail: marry_petrova@yahoo.com
Phone: +359-2-974 62 29/ 315
Professional interests: Micropropagation of medicinal plants; synthesis of secondary metabolites; cell culture; genetic transformation.

Tania Karceva, Assistant Professor
Е-mail: tania_karceva@abv.bg
Phone: +359-2-974 62 29/ 254, 205
Professional interests: Nutritive and food quality of the fruit, pleiotropic effect of mutant genes, tolerance to abiotic stress.
Research object: Solanum lycopersicum L, Capsicum annuum L., Triticum aestivum L.

Daniela Stoeva, Assistant Professor
Е-mail: dani100eva@abv.bg
Phone: +359-2-974 62 29/ 289
Professional interests: Creation and study of lines and cultivars possessing economically and biologically valuable properties; heterosis; transgression; disease resistance.
Research object: Nicotiana tabacum L.

Trendafil Nedev, Assistant Professor
E-mail: tanedev@abv.bg
Phone: +359-2-974 62 29/ 200
Professional interests: Morphogenic potential of potato, sunflower and maize; genetic transformation; in vitro micropropagation of medicinal plants; morphogenic potential of medicinal plants, long time cultivation, conservation.

Philip Philipov, Assistant
E-mail: Philipovph2003@yahoo.com
Phone: +359-2-974 62 29/ 326
Professional interests: Modeling of biotic and abiotic stress; in vitro micropropagation of medicinal plants.

Specialists: Bistra Mihailova Zornitca Stoyanova, Katalina Prokopova, Hristo Milanov Lyudmila Dimitrova Anka Petkova, Mimi Petkova, Zoya Cekova, Mariana Georgieva Mariana Traykova, Zornitca Doichinova, Krasimira Velkova – agricultural technician.

TOMATO VARIETIES

BONONYA

Bononya is a determinate cultivar with high total productivity recommended for open field production, resistant to Tm Ve and I. Fruit: 180 – 200g standard weight, fleshy, firm and smooth, uniform round in shape.

REYANA

Reyana is an indeterminate, medium early tomato hybrid for open field production and protected cropping (plastic tunnels).  Reyana produces round shaped firm and fleshy fruits, 180-200g standard weight, with a very good shelf life and flavor. Reyana is  resistant to Tomato mosaic virus (Tm-2), verticillium wilt and (Verticillium dahliae Kleb.) race 1, fusarium wilt, race 1 (Fusarium oxysporum f.sp. lycopercici (Sacc.) Snyd. and Hans.

TRAPEZITSA

Trapezitsa is a determinate cultivar for open field production, resistant to Tm Ve, I. Fruit: 160 – 180g standard weight, fleshy, firm and smooth.

 BERIKA

Berica is a determinate tomato cultivar for processing and fresh consumption. Resistant to Ve and I. Fruit: 90 – 100g standard weight, firm and smooth, characterized by relatively high lycopene content (6.5 – 7.5 mg%).

MILLA

Milla is an indeterminate, medium early cherry tomato cultivar for open field production. Milla produces cylindrical shaped firm fruits, 18-20g standard weight, resistant to cracking, characterized by 9.6% TSS and 5.1% reducing sugars content. Milla is  resistant to Tomato mosaic virus (Tm-2) and  verticillium wilt (Verticillium dahliae Kleb.) race 1.

ROSALINA ROSSA

Rosalina Rossa is an indeterminate, medium early tomato hybrid for protected cropping (plastic tunnels) and open field production. Rosalina Rossa produces round shaped firm pink fruits, 170 – 180g standard weight, resistant to cracking, with an excellent quality and storage ability. Rosalina Rossa is  resistant to Tomato mosaic virus (Tm-2), verticillium wilt (Verticillium dahliae Kleb.) race 1, fusarium wilt, race 1 (Fusarium oxysporum f.sp. lycopercici (Sacc.) Snyd. and Hans.

SWEET CORN

ZAHARINA

Zaharina is a middle-early sweet corn hybrid (FAO 450), suitable for the fresh market, canning and freezing. The plant height is 200 – 220 cm with 2 – 3 tillers. The stem is resistant of lodging.  The plants feed 2 very well-developed cobs, straight, with conic-cylindrical shape and big number of rows (18 – 20). The kernel is yellow and glossy, with tender pericarp. The hybrid is resistant to a maize dwarf mosaic (Maize dwarf mosaic virus) and a corn smut (Ustilago zeae). Average yield is 6000 – 6500 standard cobs/dka.

Sweet corn variety “Zaharina”

SESECTED PUBLICATIONS

Balacheva E, Dimitrov B, Atanassova B, Molle Е (2008) Differences in storage potential of three anthocyaninless mutants in tomato (Lycopersicon esculentum Mill.). Seed Science and Technology, 36: 730-736.

Balacheva E, Shtereva L, Atanassova B, Molle E (2008) Pleotropic effect in three anthocyaninless tomato mutants. Acta Horticulturae, 789: 41-48.

Balacheva E, Atanassova B, Hazra P (2008) Application of contemporary achievements in plant genetic improvement for tomato – the basic tool for sustainable development of agriculture in the coastal area. Journal of the Indian Society of Coastal Agricultural Research, 26: 22-27.

Balacheva E., T. Kartzeva, B. Atanassova, N. Tomlekova (2012) The diversity in tomato colours- Potential for diversity in tomato nutritive quality. Acta Horticulturae, 960: 31-35.

Doncheva S., Moustacas M., Ananieva K., Chavdarova M., Gesheva E., Vassilevska-Ivanova R (2012) Plant response to lead in the presence or absence in two sunflower genotypes (cultivated H. annuus cv. 1114 and interspecific line H. annuus x H. argophyllus ). Environmental Science and Pollution Research, 20 (2), 823-833.

Evidente, A., R. Rodeva, A. Andolfi, Z. Stoyanova, C. Perrone, A. Motta (2011) Phytotoxic polyketides produced by Phomopsis foeniculi, a strain isolated from diseased Bulgarian fennel. European Journal of Plant Pathology, 130: 173–182.

Ganeva G and V. Korzun, 2012.  Microsatellite genetic diversity analysis and  allelic variation comparison of obsolete and modern Bulgarian bread wheat (Triticum aestivum L.) varieties. Cereal Research Communications, 40 (1):14–23.

Gospodinova Z, Stoimenova E, Nikolov I, Georgieva E (2008) Distribution of (AAT) in microsatelite DNA repeated sequence in pepper lines resistant to economical diseases and comparison with some other plant families. Plant Protection (Skopje), 19:89-93.

Hazra P., Sh. Akhtar, C. Karak, P. Biswas, B. Atanassova, E. Balacheva (2012) Effect of mutant genes on the content of the nutritive quality related compounds in tomato (Solanum lycopersicum) fruits. Acta Horticulturae, 960: 311-318.

Kartzeva T, Balacheva E, Atanassova B, Molle E (2009) Effect of anthocyaninless mutant genes on the content of compounds related to nutritive quality in tomato (Solanum lycopersicum) fruits. Acta Horiculturae, 830: 285-289.

Kartzeva, T., Balacheva, E., Atanassova, B. and Molle, E (2012) Effect of three anthocyaninless genes on germination ability of the brown seed (bs) mutant in tomato. Seed Sci. & Technol., 40: 177-184.

Krasteva, L., R. Pandeva, R. Rodeva, V. Todorova, S. Neykov, K. Uzundzhalieva, N. Velcheva, D. Cvikić, E. Tome, V. Ilieva (2012) Pepper as a target object of SEE-ERA.NET project. Acta Horticulturae, 960: 151-158.

Kraptchev B, Vassilevska-Ivanova R, Velkova K (2010) Characteristic of a newBulgarian sweet corn hybrid (Zea mays var. saccharata Sturt.). Genetics and Breeding, 39 (1-2): 17-21.

Kosturkova, G., R. Rodeva, K. Tasheva, M. Dimitrova, D. Dimanov (2012) Effect of crude culture filtrates of the pathogenic fungus Phoma medicaginis on in vitro cultures of pea. Agro Life Scientific Journal, 1: 126-131.

LandjevaS., K. Kocheva, T. Karceva, A. Sepsi, I. Molnár, A. Schneider, G. Ganeva, G. Georgiev, M. Molnár-Láng (2012) Molecular cytogenetic identification of a wheat-Aegilops geniculata Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress. Plant Breeding, 131(1): 81-87.

Landjeva S., KarcevaT., KorzunV., GanevaG (2012) Seedling growth under osmotic stress and agronomic traits in Bulgarian semi-dwarf wheat – comparison of genotypes with Rht8 and/or Rht-B1 genes. Crop and Pasture Science, 62 (12): 1017-1025.

Naidenova N., Vassilevska-Ivanova R (2008) New mutant line in pea (Pisum sativum L.) characterizing by funnel leaflets, pin/needle structures and flower abnormalities. Journal of Genetics and Breeding, (Italy), 62 (1-4): 59-66.

Naidenova N., Vassilevska-Ivanova R (2009) Supaeromaculata lines in pea (Pisum sativum L.) obtained following mutagenesis. The Open Horticulture Journal, 2: 43-48.

Nikolova M., Petrova M., Zayova E., Vitkova A.,Evstatieva L (2013) Comparative study of in vitro, ex vitro and in vivo grown plants of Arnica montana – polyphenols and free radical scavenging activity, Acta Botanica Croatica, 72 (1): 13-22.

Petrova M., E. Zayova, E. Yankova, G. Baldzhiev (2011) Plant regeneration from callus culture of Arnica montana. Romanian Biotechnological Letters, 16 (1): 92-97.

Petrova M., E. Zayova,A. Vitkova (2011) Effect of silver nitrate on in vitro root formation of Gentiana lutea. Romanian Biotechnological Letters, 16 (1): 53-58.

Petrova M, Zayova E., Vassilevska-Ivanova R., Vlahova M (2012) Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L., Acta Physiologiae Plantarum, 34 (2): 1597–1606.

Petrova, M., Zayova, E., Todorova, M., Staneva, J., Vitkova, A. and Evstatieva, L N (2012) Sesquiterpene lactones contents in multiple in vitro shoots of three Arnica montana populations. Acta Horticulturae, 955: 93-99.

Petrova M., Zayova E., Vlahova M (2013) Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes. Central European Journal of Biology, 8 (5): 470-479.

Petrova D, Marinova G, Stoimenova E, Kapchina-Toteva V (2008) Influence of cucumber mosaic virus on some physiological and biochemical indices of resistant and susceptible to virus pepper. Oxidation Communications, 31: 730-736.

Petrova D., G. Chaneva, E. Stoimenova, V. Kapchina-Toteva (2012) Effect of cucumber mosaic virus on the contents of chlorophyll, proline, the degree of lipid peroxidation and phenotypic expression of pepper lines with different susceptibility to virus, Oxidation Communications, 35: 182-189.

Radkova M, Balacheva E, Atanassova B,Iantcheva A, Atanassov A(2009) Study on the potential of genic male sterility in tomato as a tool for pollen flow restriction. Biotechnology and Biotechnological Equipment, 23 (3): 1303-1307.

Rodeva R, Gabler J, Stoyanova Z (2009) First evidence of Itersonilia perplexans on dill (Anethum graveolens) in Bulgaria. Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture, 28: 193-198.

Rodeva R, Ivanova B, Stoyanova Z, Stefanov D, Manеva S (2009) Resistance components to Septoria lycopersici in tomato. Acta Horticulturae, 808: 45-49.

Rodeva R, Pandeva R, Stoyanova Z (2009) A new fruit disease of pepper in Bulgaria caused by Phomopsis capsici. Acta Horticulturae, 830: 551-556.

Rodeva R, Stoyanova Z, Pandeva R, Petrov N (2009) Field reaction to anthracnose caused by Colletotrichum spp. on pepper fruits. Acta Horticulturae, 830: 557-562.

Rodeva R, Gabler J (2011) Umbel browning and stem necrosis: a new disease of fennel in Bulgaria. Journal of Phytopathology, 159: 184-187.

Rodeva, R., D. Kostova, P. Chavdarov, M. Mijatovic, J. Merkuri, M. Cara, G. Pasev, Z. Stoyanova, I. Karov, S. Mitrev, B. Kovacevik, S. Goudoudaki, I. Manoussopoulos (2012)  Pepper diseases in Balkan region. Acta Horticulturae, 960: 365-370.

Shtereva L, Atanassova B, Karcheva T, Petkov V (2008) The effect of water stress on the growth rate, water content and proline accumulation in tomato calli and seedlings. Acta Horticulturae, 789: 189-198.

Stefanov D., E. Stoimenova, G. Marinova, B. Ivanova A. Edreva. (2012) Accelerated leaf senescence takes part in enhanced resistance in cucumber mosaic virus inoculated pepper leaves. Acta Physiologiae Plantarum, 34: 181-190.

Stoimenova E, Bogatzevska N (2009) Systemic acquired resistance induced by salicylic acid and tomato mosaic virus against bacterial spot and speck diseases in tomato. Plant Protection (Skopje), 20: 100-104.

Stoyanova, Z., R. Rodeva, I. Karov, B. Kovacevik, V. Manova, R. Georgieva (2013) Morphological and molecular characterization of Colletotrichum coccodes isolated from pepper cultivated in Bulgaria and Macedonia. J. Nat. Sci., Matica Srpska Novi Sad, 124: 249-262

Tomlekova Nasya,  Svetla Yancheva, Elena Balacheva, Bistra Atanasova (2012) Molecular Identification of Tomato Mutant Lines. Bioremediation, Biodiversity and Bioavailability, Global Science Books, 6: 58-64.

Vassilevska-Ivanova R., Kraptchev B., Stancheva I., Geneva M (2012) Agronomic characteristic and antioxidant activity and of an interspesific hybrid line between Helianthus annuus and Helianthus mollis. Compt. Rend. ABS, 65 (9): 1211-1218.

Vassilevska-Ivanova R., Kraptchev B., I. Stancheva, M. Geneva (2013) A compact sunflower line produced after cross Helinathus annuus x Verbesina encelioides. Central European Journal of Biology, 8 (5), 492-498.

Yankova Е., G. Baldzhiev, M. Petrova, E. Zayova, P. Yurukova (2010) Analyses on pollen and seed productivity and effectiveness in Gentiana lutea L., Biotechnol. & Biotechnol. Eq, 24: 45-48.

Zayova E., Vassilevska-Ivanova R., Stoeva D., Kraptchev B (2010) Somaclonal variation through indirect organogenesis in eggplant (Solanum melongena L.). Biological Diversity and Conservation, 3 (3): 1-5.

Zayova Е., M. Petrova, Vassilevska-Ivanova R., Nedev T (2010) Micro propagation of Valeriana officinalis trough tissue culture, Compt. Rend ABS, 63 (12): 1749-1756.

Zayova E., Stancheva I., Geneva M., Petrova M., Vasilevska-Ivanova R (2012) Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants. Central European Journal of Biology, 7 (4): 698-707.

Zayova E., Vassilevska-Ivanova R., Kraptchev B., Stoeva D (2012) Indirect shoot organogenesis of eggplant (Solanum melongena L.), Journal of Central European Agriculture, 13 (3): 446-457.

Zayova E., Stancheva I., Geneva M., Petrova M., Dimitrova L (2012) Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turk J Biol, 36, 2012, 1-10.

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